| Total | Core | Buffer | Transition | |||||
| Biosphere Reserve | Acres | Area(s) | Zone(s) | Area(s) | ||||
| Aleautian Island | 2,720,430 | 2,720,430 | - | - | ||||
| Big Bend | 699,903 | - | - | - | ||||
| Cascade Head | 17,423 | - | - | - | ||||
| Central Plains | 15,345 | - | - | - | ||||
| Channel Islands | 1,185,220 | - | - | - | ||||
| Coram | 7,460 | - | - | - | ||||
| Denali | 1,932,322 | - | - | - | ||||
| Desert | 55,630 | 1,680 | - | - | ||||
| Everglades & Dry Tortugas | 1,572,572 | 1,326,280 | 246,292 | - | ||||
| Fraser | 23,049 | - | - | - | ||||
| Glacier | 1,013,248 | - | - | - | ||||
| H.J. Andrews | 15,814 | - | - | - | ||||
| Hubbard Brook | 7,808 | - | - | - | ||||
| Jornada | 193,472 | - | - | - | ||||
| Luquillo | 8,616 | - | - | - | ||||
| Noatak | 7,499,979 | - | - | - | ||||
| Olympic | 922,662 | - | - | - | ||||
| Organ Pipe Cactus | 330,822 | - | - | - | ||||
| Rocky Mountain | 263,680 | - | - | - | ||||
| San Dimas | 17,161 | - | - | - | ||||
| San Joaquin | 4,527 | - | - | - | ||||
| Sequoia-King Canyon | 863,721 | - | - | - | ||||
| Stanislaus-Tuolumne | 1,500 | - | - | - | ||||
| Three Sisters | 199,904 | - | - | - | ||||
| Virgin Islands | 15,147 | - | - | - | ||||
| Yellowstone | 2,219,820 | - | - | - | ||||
| Beaver Creek | 275,022 | - | - | - | ||||
| Konza Prairie | 8,616 | - | - | - | ||||
| Niwot Range | 2,965 | - | - | - | ||||
| University of Michigan Biological Station | 10,003 | - | - | - | ||||
| Virginia Coast | 33,386 | - | - | - | ||||
| Hawaiian Islands | 245,976 | - | - | - | ||||
| Isle Royale | 571,787 | - | - | - | ||||
| Big Thicket | 96,668 | - | - | - | ||||
| Guanica | 9,884 | - | - | - | ||||
| California Coast Range | 153,444 | - | - | - | ||||
| Central Gulf Coast Plain | 40,529 | - | - | - | ||||
| South Atlantic Coastal Plain | 20,317 | - | - | - | ||||
| Mojave and Colorado Deserts | 5,901 | 5,901 | - | - | ||||
| Carolinian-South Atlantic | 310,222 | - | - | - | ||||
| Glacier Bay-Admiralty Is. | 3,743,602 | - | - | - | ||||
| Golden Gate | 523,906 | - | - | - | ||||
| New Jersey Pinelands | 1,082,817 | - | - | - | ||||
| Southern Appalachian | 37,547,688 | 581,528 | - | 36,966,160 | ||||
| Champlain-Adirondak | 9,859,290 | 2,372,160 | 2,792,230 | 4,694,900 | ||||
| Mammoth Cave Area | 909,276 | - | - | - | ||||
| Land Between The Lakes Area | 170,005 | - | - | - | ||||
| Total Acres | 77,428,540 | 7,007,978 | 3,038,522 | 41,661,060 | ||||
| * Transition Area acreages reported when given by State | ||||||||